BarleyMax is a premium, uncooked, natural barley grass juice powder. The advantages of consuming BarleyMax go approach past simply the nutritional vitamins and minerals listed on the Diet Details panel. The testing reported right here reveals that BarleyMax prevents DNA harm. A Comet assay of colon most cancers cells discovered {that a} dilute resolution of BarleyMax was in a position to defend the cells from DNA harm induced by hydrogen peroxide.
Why You Ought to Care About DNA Harm
There are a number of explanation why DNA harm needs to be prevented. Mutations in DNA, particularly within the DNA controlling cell progress, can result in errors that will trigger ailments like most cancers. Broken DNA can cease cells from working easily, resulting in organ dysfunction, failure and accelerated ageing. Your physique has restore mechanisms to repair broken DNA, however you possibly can overwhelm the system and even harm the DNA encoding the restore mechanisms.
How Your Physique Prevents DNA Harm
Our our bodies have a three-fold protection in opposition to DNA harm—enzymes which disarm free radicals, small molecules which soak up free radicals (like uric acid), and dietary antioxidants to spice up safety of DNA, lipids, and proteins.
What’s Oxidative Harm?
The cells of our our bodies are continuously bombarded with free radicals produced from the “hearth” of oxygen-based metabolism. These free radicals trigger organic “rust,” making the techniques of the physique seize up and grind to a halt. Free radical harm performs a significant position in illness and premature deaths.
How the Research was Performed
On this examine, the HT29 cell line, a human colorectal adenocarcinoma cell line broadly used as a colon most cancers mannequin, was chosen. HT29 cells have been incubated with BarleyMax at numerous concentrations together with hydrogen peroxide for 1 hour. After thorough rinsing the cells have been blended with agarose gel and laid onto a microscope slide. Cells have been then lysed, electrophoresed, the DNA was stained, after which particular person cells have been scored. Broken DNA is shorter than intact DNA and strikes faster beneath the electrophoresis subject, forming a tail that seems like a comet when visualized with fluorescent dye. The quantity of DNA within the tail is quantified in comparison with the quantity remaining inside the cell nucleus. A much bigger tail signifies larger DNA harm.
The Outcomes of the Research
As proven in Determine 1, BarleyMax at 0.03% considerably reduces DNA harm. At 0.25% BarleyMax or larger the cells have been virtually utterly protected; harm was lowered to about 10% of the damaging management worth.
This Form of End result is Additionally Seen with Different Meals
This was an in vitro experiment, so the information can’t be instantly utilized to DNA safety in folks. The Comet assay is broadly used and accepted as a really delicate methodology to detect DNA harm in single cells. In vitro outcomes like these with BarleyMax do correlate effectively with DNA safety of white blood cells in volunteers who devour kiwifruit juice (1), and broccoli sprouts (2). Additionally, the Comet assay was in a position to detect DNA protecting results of consuming spinach and tomato puree (3), blood orange juice (4) and carrot juice (5). Complete food regimen results may even be captured. The fecal water of volunteers who ate a food regimen excessive in fats and meat, when in comparison with a low-fat, low-meat, high-fiber food regimen, precipitated twice as a lot DNA harm in a Comet assay (6).
Conclusion
So, BarleyMax has DNA-protective properties, probably by a number of mechanisms. For maximal safety an optimum food regimen and life-style should be adopted. BarleyMax could be a part of that safety.
What’s BarleyMax?
BarleyMax is uncooked, natural barley grass juice powder, from younger barley grass and alfalfa. It’s the juice from a complete meals, replete with the entire nutritional vitamins, minerals, antioxidants, and enzymes of those very potent inexperienced meals. It has been rigorously processed in order that as a lot as doable of the vibrancy, style, and lifetime of the contemporary juice is preserved for handy consumption.
References
- Collins BH, Horska A, Hotten PM, Riddoch C, Collins AR (2001) Kiwifruit protects in opposition to oxidative DNA harm in human cells and in vitro. Nutr Most cancers 39:148–53. doi: 10.1207/s15327914nc391_20
- Gill CIR, Haldar S, Porter S, Matthews S, Sullivan S, Coulter J, et al (2004) The impact of cruciferous and leguminous sprouts on genotoxicity, in vitro and in vivo. Most cancers Epidemiol Biomarkers Prev 13:1199–205. doi: 10.1158/1055-9965.1199.13.7
- Porrini M, Riso P, Oriani G (2002) Spinach and tomato consumption will increase lymphocyte DNA resistance to oxidative stress however this isn’t associated to cell carotenoid concentrations. Eur J Nutr 41:95–100. doi: 10.1007/s003940200014
- Guarnieri S, Riso P, Porrini M (2007) Orange juice vs vitamin C: impact on hydrogen peroxide-induced DNA harm in mononuclear blood cells. Br J Nutr 97:639–643. doi: 10.1017/S0007114507657948
- Lee H-J, Park YK, Kang M-H (2011) The impact of carrot juice, β-carotene supplementation on lymphocyte DNA harm, erythrocyte antioxidant enzymes and plasma lipid profiles in Korean smoker. Nutr Res Pract 5:540–547. doi: 10.4162/nrp.2011.5.6.540
- Rieger MA, Parlesak A, Pool-Zobel BL, Rechkemmer G, Bode C (1999) A food regimen excessive in fats and meat however low in dietary fibre will increase the genotoxic potential of “faecal water.” Carcinogenesis 20:2311–6. doi: 10.1093/carcin/20.12.2311
Clarification of the Determine
Broken DNA is shorter than intact DNA and strikes faster beneath the electrophoresis subject, forming a tail that seems like a “comet” when visualized with fluorescent dye. A much bigger tail signifies larger DNA harm; that is indicated by the peak of the bar on the graph; the taller the bar, the extra DNA harm.
- The primary column is a damaging management, a “car” management; the experiment process itself doesn’t induce noticeable DNA harm.
- The second column is a optimistic management, the place the cells are incubated with hydrogen peroxide. Focus of the hydrogen peroxide is 200 micromolar. Clearly there’s plenty of DNA harm.
- The following columns are cells incubated with the identical quantity of hydrogen peroxide and growing quantities of BarleyMax.
- As the quantity of BarleyMax was elevated the cells have been protected an increasing number of till there was virtually no harm of the DNA, in comparison with the “car” management.
- Every remedy with BarleyMax is twice as a lot as the following column on the left.
